Particle Tracking and Robust Estimation of Azimuthal Averages from Confocal Laser Scanning Microscopy Data

TitleParticle Tracking and Robust Estimation of Azimuthal Averages from Confocal Laser Scanning Microscopy Data
Publication TypeConference Paper
Year of Publication2008
Authorsvon Lieres, E, Niehoff, C, Nöh, K
Conference Name5th European Congress on Computational Methods in Applied Sciences and Engeineering - ECCOMAS
Conference LocationVenice, Italy
Abstract

Confocal laser scanning microscopy is a powerful tool for in situ observation of biomolecule chromatography. A typical experimental set up consists of a transparent micro column that is packed with porous beads and placed under the microscope. The void fraction of the column and the particle pores are initially filled with liquid buffer solution. The buffer is then pumped through the column and temporarily enriched with particular biomolecules, whose concentrations are monitored with the microscope. The molecules are transported through the column by convection and into the particle pores by diffusion.