Correlation between growth rates, EIIACrr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12.

TitleCorrelation between growth rates, EIIACrr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12.
Publication TypeJournal Article
Year of Publication2007
AuthorsBettenbrock, K, Sauter, T, Jahreis, K, Kremling, A, Lengeler, JW, Gilles, E-D
JournalJ Bacteriol
Volume189
Issue19
Pagination6891-900
Date Published2007 Oct
ISSN0021-9193
KeywordsBioreactors, Cyclic AMP, Cyclic AMP Receptor Protein, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Glucose, Mannitol, Phosphoenolpyruvate Sugar Phosphotransferase System, Phosphorylation, Promoter Regions, Genetic, Sucrose
Abstract

In Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion mediated by phosphoenolpyruvate-dependent protein kinase enzyme IIA(Crr) (EIIA(Crr)) (= EIIA(Glc)) and catabolite repression mediated by the global regulator cyclic AMP (cAMP)-cAMP receptor protein (CRP). We measured in a systematic way the relation between cellular growth rates and the key parameters of catabolite repression, i.e., the phosphorylated EIIA(Crr) (EIIA(Crr) approximately P) level and the cAMP level, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources or by growing the cells with limited concentrations of glucose, sucrose, and mannitol in continuous bioreactor experiments. The ratio of EIIA(Crr) to EIIA(Crr) approximately P and the intracellular cAMP concentrations, deduced from the activity of a cAMP-CRP-dependent promoter, correlated well with specific growth rates between 0.3 h(-1) and 0.7 h(-1), corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system.

DOI10.1128/JB.00819-07
Alternate JournalJ. Bacteriol.