The YmdB phosphodiesterase is a global regulator of late adaptive responses in Bacillus subtilis.

TitleThe YmdB phosphodiesterase is a global regulator of late adaptive responses in Bacillus subtilis.
Publication TypeJournal Article
Year of Publication2014
AuthorsDiethmaier, C, Newman, JA, Kovács, ÁT, Kaever, V, Herzberg, C, Rodrigues, C, Boonstra, M, Kuipers, OP, Lewis, RJ, Stülke, J
JournalJ Bacteriol
Volume196
Issue2
Pagination265-75
Date Published2014 Jan
ISSN1098-5530
KeywordsBacillus subtilis, Biofilms, Crystallography, X-Ray, DNA Mutational Analysis, Gene Deletion, Gene Expression Regulation, Bacterial, Models, Molecular, Phosphoric Diester Hydrolases, Protein Conformation
Abstract

Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and are deficient in SlrR, a SinR antagonist. Here, we report the functional and structural characterization of YmdB, and we find that YmdB is a phosphodiesterase with activity against 2',3'- and 3',5'-cyclic nucleotide monophosphates. The structure of YmdB reveals that the enzyme adopts a conserved phosphodiesterase fold with a binuclear metal center. Mutagenesis of a catalytically crucial residue demonstrates that the enzymatic activity of YmdB is essential for biofilm formation. The deletion of ymdB affects the expression of more than 800 genes; the levels of the σ(D)-dependent motility regulon and several sporulation genes are increased, and the levels of the SinR-repressed biofilm genes are decreased, confirming the role of YmdB in regulating late adaptive responses of B. subtilis.

DOI10.1128/JB.00826-13
Alternate JournalJ. Bacteriol.