The YmdB phosphodiesterase is a global regulator of late adaptive responses in Bacillus subtilis.
Title | The YmdB phosphodiesterase is a global regulator of late adaptive responses in Bacillus subtilis. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Diethmaier, C, Newman, JA, Kovács, ÁT, Kaever, V, Herzberg, C, Rodrigues, C, Boonstra, M, Kuipers, OP, Lewis, RJ, Stülke, J |
Journal | J Bacteriol |
Volume | 196 |
Issue | 2 |
Pagination | 265-75 |
Date Published | 2014 Jan |
ISSN | 1098-5530 |
Keywords | Bacillus subtilis, Biofilms, Crystallography, X-Ray, DNA Mutational Analysis, Gene Deletion, Gene Expression Regulation, Bacterial, Models, Molecular, Phosphoric Diester Hydrolases, Protein Conformation |
Abstract | Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and are deficient in SlrR, a SinR antagonist. Here, we report the functional and structural characterization of YmdB, and we find that YmdB is a phosphodiesterase with activity against 2',3'- and 3',5'-cyclic nucleotide monophosphates. The structure of YmdB reveals that the enzyme adopts a conserved phosphodiesterase fold with a binuclear metal center. Mutagenesis of a catalytically crucial residue demonstrates that the enzymatic activity of YmdB is essential for biofilm formation. The deletion of ymdB affects the expression of more than 800 genes; the levels of the σ(D)-dependent motility regulon and several sporulation genes are increased, and the levels of the SinR-repressed biofilm genes are decreased, confirming the role of YmdB in regulating late adaptive responses of B. subtilis. |
DOI | 10.1128/JB.00826-13 |
Alternate Journal | J. Bacteriol. |