Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum.
Submitted by rmuench on Thu, 09/12/2013 - 11:32
| Title | Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum. |
| Publication Type | Journal Article |
| Year of Publication | 2013 |
| Authors | Hentschel, E, Will, C, Mustafi, N, Burkovski, A, Rehm, N, Frunzke, J |
| Journal | Microb Biotechnol |
| Volume | 6 |
| Issue | 2 |
| Pagination | 196-201 |
| Date Published | 2013 Mar |
| ISSN | 1751-7915 |
| Keywords | Amino Acid Sequence, Bacterial Proteins, Biotechnology, Corynebacterium glutamicum, Gene Expression Regulation, Bacterial, Genes, Reporter, Genetic Variation, Genetic Vectors, Luminescent Proteins, Molecular Sequence Data, Promoter Regions, Genetic, Protein Stability, RNA-Binding Proteins |
| Abstract | Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum. |
| DOI | 10.1111/j.1751-7915.2012.00360.x |
| Alternate Journal | Microb Biotechnol |