Antibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis.

TitleAntibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis.
Publication TypeJournal Article
Year of Publication2004
AuthorsMascher, T, Zimmer, SL, Smith, T-A, Helmann, JD
JournalAntimicrob Agents Chemother
Volume48
Issue8
Pagination2888-96
Date Published2004 Aug
ISSN0066-4804
KeywordsAnti-Bacterial Agents, Bacillus subtilis, Blotting, Northern, Cell Membrane, Chromosome Mapping, Culture Media, DNA, Bacterial, DNA Primers, Drug Evaluation, Preclinical, Lac Operon, Lipid Metabolism, Membrane Lipids, Operon, Plasmids, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction
Abstract

Soil bacteria are among the most prodigious producers of antibiotics. The Bacillus subtilis LiaRS (formerly YvqCE) two-component system is one of several antibiotic-sensing systems that coordinate the genetic response to cell wall-active antibiotics. Upon the addition of vancomycin or bacitracin, LiaRS autoregulates the liaIHGFSR operon. We have characterized the promoter of the lia operon and defined the cis-acting sequences necessary for antibiotic-inducible gene expression. A survey for compounds that act as inducers of the lia promoter revealed that it responds strongly to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin, and vancomycin). Chemicals that perturb the cytoplasmic membrane, such as organic solvents, are also weak inducers. Thus, the reporter derived from P(liaI) (the liaI promoter) provides a tool for the detection and classification of antimicrobial compounds.

DOI10.1128/AAC.48.8.2888-2896.2004
Alternate JournalAntimicrob. Agents Chemother.